Quantification of human immunodeficiency virus type-1 (HIV-1) proviral DNA is increasingly used to measure the HIV-1 cellular reservoirs, a helpful marker to evaluate the efficacy of antiretroviral ...

The polymerase chain reaction (PCR), invented about three decades ago, soon entered mainstream use thanks to an ongoing series of refinements. One particularly important refinement, introduced about ...

Following real-time RT-PCR, each amplification product was diluted 10-fold with water and 0.5 μl the diluted products were then subjected to capillary electrophoresis in an ABI Prism 3100 Genetic ...

Real-time PCR or quantitative PCR (Polymerase Chain Reaction) is an advancement of the standard PCR technique, which enables continuous monitoring of the reaction and collection of data in real time.

The changing technology landscape will affect the BioWatch program. This chapter comments on additional assay performance considerations that arise from the use of multiplexed real-time polymerase ...

In recent years, quantitative real-time PCR tests have been extensively developed in clinical microbiology laboratories for routine diagnosis of infectious diseases, particularly bacterial diseases.

The OpenArray® Real-Time PCR Platform puts the control in the hands to get the answers you need. It delivers the best combination of convenience, flexibility, throughput, and cost-effectiveness for ...

The real-time polymerase chain reaction( PCR) has become a key tool for molecular assay development, expanding into applications as diverse as in vitro diagnostics (IVDs), food safety testing, and ...

Polymerase chain reaction (PCR) is a fundamental molecular biology tool that scientists use to amplify and analyze genetic material, such as DNA and RNA. PCR involves the enzymatic amplification of ...

Alexander Edwards receives funding from the EPSRC, the Wellcome Trust, and the Newton Fund. Alexander Edwards is co-founder, director and shareholder in Capillary Film Technology Ltd who develop blood ...

Expert Rev Mol Diagn. 2012;12(7):731-754. SYBR Green and dual-hybridization probes are often used for melting point analysis at the end of the amplification. qPCR presents a number of advantages over ...